As an antibacterial drug, 4-quinolones, also known as pyridone or pyridone, are artificially synthesized antibacterials containing the basic structure of 4-quinolone, which have a bactericidal effect on a variety of Gram-negative bacteria. For the treatment of genitourinary diseases, gastrointestinal diseases, and Gram-negative bacterial infections of the respiratory tract and skin tissues, commonly used drugs are norfloxacin, ofloxacin, ciprofloxacin, fleroxacin and the like.

Quinolone antibiotics are a class of drugs commonly used in humans and animals. Because of its broad antibacterial spectrum, strong antibacterial activity, no cross-resistance and toxic side effects with other antibacterial drugs, it is widely used in animal husbandry, aquatic products and other aquaculture, including poultry, livestock, aquatic products, etc. For disease prevention and treatment.

Residues of quinolones in animal tissues. After long-term consumption of animal foods containing Chinese medicines containing lower concentrations of QNs, Chinese herbal medicines, etc., quinolones antibiotics accumulate in the human body, causing serious diseases of the human body. Drug resistance.

Eggs and egg products are particularly common foods in human daily life, and the quinolone antibiotics contained in this test are not particularly deep in the present. This paper uses LC-600B isocratic liquid chromatograph in conjunction with relevant literature. (Tengzhou Luchuang Analytical Instrument Co., Ltd.), equipped with a fluorescence detector, using solid phase extraction method and high-performance liquid phase fluorescence detection method to determine the content of quinolone antibiotics contained in eggs.

Sample treatment: Take 2 eggs and crush, mix the egg white and egg yolk evenly, and store in -20 °C refrigerator. Accurately weigh 2g of homogenized eggs into a 50mL centrifuge tube, add 20mL tissue extract (0.1mol / L phosphate buffer, PH = 7.0), vortex and mix, centrifuge at 4000r / min for 10min, will be on The supernatant was transferred to another centrifuge tube, 20 mL of water-saturated n-hexane was added, vortexed, centrifuged at 4000 r/min for 10 min, and the upper layer was discarded. Further, 20 mL of water-saturated n-hexane was added to the lower layer solution, and the mixture was shaken and centrifuged, and the upper layer solution was carefully sucked by a pipette, and the lower layer was a solid phase extraction sample solution.

Instrument conditions:

Spectral analysis: