Classification and application of Semrock filters

Founded in 2000, Semrock Inc. is a manufacturer of high-end filters for biotechnology and analytical instrumentation, including: fluorescence filters, Raman filters, laser filters, and microscope filters. Semrock uses ion beam sputtering to produce hard-film filters that offer breakthrough performance in terms of transmission, OD, lifetime, and stability. It has been applied to top scientific research laboratories and cutting-edge optical instruments all over the world. New Force Optoelectronics (Hangzhou) is responsible for introducing its products to China and promoting and applying it to various high-end customers.

Semrock is part of the IDEX Group of the United States and is recognized as a world leader in high quality optical filters and coatings. Semrock offers a wide range of standard and custom optical components including narrowband filters, high pass filters, low pass filters, neutral attenuators, dichroic filters, etc., with extremely narrow bandwidth And excellent cut-off depth, transflective efficiency >90%. The hard-film technology of ion beam sputtering ensures that its optical properties are equally excellent in high-humidity environments.

Fluorescent filter

Fluorescence spectroscopy: The absorption spectrum of the molecule in the absorption band, and then the long-wave radiation spectrum, ie the fluorescence spectrum, is emitted in the emission band. In the field of analysis, fluorescence can be used for qualitative, quantitative, and real-time observation of biological and chemical activities. Fluorescence is widely used in biology and analysis due to its high sensitivity, specificity and simplicity.

Most fluorescent instruments, including fluorescence microscopes, are based on fluorescence filters . A typical system consists of three filters: an excitation filter (excitation), a dichroic beam splitter (split), and a radiation filter (radiation). The excitation filter, typically a bandwidth filter, is used to pass the fluorescence absorption spectrum. The two-color beam mirror is an edge filter that is typically assembled at a 45° angle for reflecting excitation and transmitting radiation. Radiation filters, typically bandwidth filters, are only permeable to radiant light to filter out other spectra, including excitation light.

When the sample to be tested has a multi-fluorescence spectrum, the narrow band filter is the first solution. The narrow-band filter only allows the fluorescence spectrum of the strongest radiation to be reduced, reducing auto-fluorescence noise and interaction effects, thereby increasing the signal-to-noise ratio of the system. If the sample has sufficient fluorescent signal, a narrow band filter is ideal.



Raman filter

Raman spectroscopy detects and discriminates by vibration and rotation of molecular energy level structures. Unlike fluorescence spectra, which require fluorescence to match the molecular properties, Raman spectroscopy directly detects molecules without chemical changes. In addition, the Raman spectral information is a few percent of the excitation source and is orders of magnitude weaker than the fluorescent signal. Therefore, lasers are generally used for Raman spectroscopy to increase signal strength. At the same time, high-sensitivity detectors are used to obtain very weak signals.

In order to prevent the laser from reaching the detector (submerging the weak Raman spectral signal), the new force optoelectronics supplies a notch filter to filter the laser spectrum to ensure that both ends of the spectrum are permeable.



Laser filter

Lasers have unique characteristics, including: narrow bandwidth, high intensity, high brightness, and coherence, which are widely used in scientific research, biomedical, industrial processing, material processing and other fields. Thin film dielectric filters with high transmission, deep blocking, steep edges, high damage threshold, etc., so the laser filter is an important optical component in the laser system.

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