Rapid determination of pepper seed bud rate by towel roll
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September 03 08:42:53, 2025
According to the National Agricultural Crop Inspection Regulations (GB3543-83), pepper seed germination tests typically use gauze or filter paper as the germination bed. The germination potential is determined within 6 days, while the germination rate is measured after 14 days. However, using gauze or filter paper can be challenging due to difficulty in moisture control, which may lead to seed rot and longer testing times. In contrast, the towel roll method offers a simpler, faster, and more accurate alternative. It allows for germination potential to be assessed in just 4 days and germination rate in 7 days, with an error margin of less than 2% compared to the standard method. This makes it an ideal choice for rapid seed germination testing in indoor pepper seed inspections.
The procedure is straightforward:
1. First, prepare clean towels by boiling and sterilizing them. Once cooled to room temperature, wring them out until no water drips.
2. Take four random samples from the seeds that have passed the cleanliness test, with each sample containing 100 seeds, totaling 400 seeds.
3. Place four sterilized, damp towels on a flat surface. Arrange the seeds evenly on one half of the towel, ensuring there's at least the same size gap between each seed. Leave about 1.5 cm of space around the edges. Cover the seeds with the other half of the towel, insert a bamboo chopstick in the center, and carefully roll the towel into a tight stick. Secure both ends with rubber bands and place the roll in a sealed plastic bag. Label the bag with the date, type of seed, and number of repetitions. Repeat this process four times and place them in a 30°C germination chamber. No additional water should be added during the entire process.
4. On the fourth and seventh days of the test, examine the seeds for germination. Calculate the average number of germinated seeds to determine the germination potential and germination rate for the batch.
When evaluating germination, all healthy seedlings—excluding broken ones—are counted as normal. All other criteria follow the same standards as the traditional method. This approach ensures reliable and efficient results while maintaining consistency with established agricultural practices.