Wuzhishan miniature pig experimental inbred line was successfully developed
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March 28 11:30:17, 2019
A few days ago, the "Five-finger Pigs Experimental Inbreeding Lines and Molecular Genetics Basic Research" project completed under the auspices of the Animal Husbandry Institute of the Chinese Academy of Agricultural Sciences passed the results appraisal organized by the Ministry of Agriculture. This project takes the Wuzhishan miniature pig (WZSP), a rare species in China, as the material, and after inbreeding and breeding for 17 generations, has cultivated the first batch of inbred lines for experimental Wuzhishan miniature pig (WZSP) in the world, and has established more than 100 inbred basic groups. head. Tests have shown that the physiological and biochemical values ​​of blood and the metabolism of the heart, liver, and kidney are similar to humans, and the blood type is similar to humans. type. Using comparative studies of polymorphic microsatellite probes and skin grafts, it was confirmed that the homozygosity of alleles in the population increased with the increase in inbreeding generations. Among them, Line I has been acquired for 17 generations, and Line II has been bred for 16 generations. Nine polymorphic probes confirmed that F15, populations SW1377, SW2409 and other five sites had reached 100% homozygosity, and their heterozygosity and average polymorphism information content were 0.2075 and 0.1588, respectively. The expert group unanimously agreed that the overall research level of this achievement has reached the international advanced level, and the cultivation of inbred lines is at the international leading level.