Human stable osteocalcin (1-43/49) specific enzyme free kit
Human Stable Osteocalcin ( 1-43/49 ) Specific Enzyme Free Kit [ intended use] This ELISA kit is used to quantify the concentration of human osteocalcin ( 1-43 ) and osteocalcin ( 1-49 ) (also known as N- terminal and middle osteocalcin or stable osteocalcin). . The test can be used to detect human bone formation activity or osteoblast activity, which is associated with changes in bone turnover rate in metabolic bone diseases such as osteoporosis, primary hyperparathyroidism, hyperthyroidism , deformity osteitis and renal osteodystrophy. [ Introduction] Osteocalcin [ also known as osteocalcin ( BGP ) ] is a major non-collagen protein in bone and dentin. The composition of osteocalcin includes vitamin K and vitamin D3 . The newly synthesized osteocalcin is partially released into the bloodstream and partially infiltrated into the bone matrix. Osteocalcin ( 1-49 ) and its fragments, as well as osteocalcin ( 1-43 ), are released into the bloodstream. In the blood circulation, liver and kidney, osteocalcin ( 1-49 ) degrades to produce stable serum osteocalcin ( 1-43 ). Stable serum osteocalcin ( 1-43 ) can also be produced when a human sample is taken for in vitro degradation because the six amino acids at the C- terminus of osteocalcin ( 1-49) are unstable, and in vitro, these six amino acids are extremely Easy to fall off at room temperature. Some studies have shown that the clinical determination of stable osteocalcin [ osteocalcin (1-43/49)] is more effective, it can provide a more accurate assessment of bone turnover rate. Osteocalcin is produced by osteoblasts, which are often used as a biochemical or biological indicator during bone formation. When osteoporosis is treated with bone-forming drugs ( such as Forteo) , it is generally observed that a stable positive serum osteocalcin level has a strong positive correlation with an increase in bone density. In many drug treatment studies, osteocalcin is the preferred biochemical indicator for the tracking and assessment of specific effects of bone formation. [ Test principle] The purpose of this ELISA design, development and production is to quantify stable human osteocalcin ( 1-43 ) and osteocalcin ( 1-49 ) in serum or plasma samples . The kit is based on a "two-site sandwich" technique that uses two specific antibodies to bind to different epitopes of human osteocalcin. Standards, controls, and samples to be tested are added to streptavidin-coated microplates. Subsequently, a biotin-conjugated human osteocalcin N- terminal specific polyclonal antibody and a peroxidase-labeled human osteocalcin 20-43 region-specific monoclonal antibody mixture were added. After the first incubation period, a " two-site sandwich " structure of "biotin antibody - human osteocalcin- HRP monoclonal antibody" was formed , which is specific for "streptavidin- biotin" "Affinity binding is adsorbed to the inner wall of the microplate. The free monoclonal antibody and buffer matrix are washed away by the detergent. The microplate was added with a peroxidase substrate ( 3,3',5,5' -tetramethylbenzidine, TMB ) and the absorbance was measured after a period of reaction. The enzymatic activity of the immune complex on the inner wall of the microplate is directly proportional to the concentration of human osteocalcin in the sample. According to the absorbance of different human osteocalcin concentrations in the standard, the standard curve is drawn according to the "point to point" or " 4- parameter method", and the osteocalcin concentration in the sample to be tested can be directly obtained from the standard curve according to the measured absorbance. Brief experimental steps High Purity Sodium Dodecyl Sulfate Toothpaste Base Ingredients,Detergent Making Ingredients,Detergent Recipe Ingredients,High Purity Sodium Dodecyl Sulfate XINGZHILIAN BIOLOGICALR&D CO.,LTD , https://www.xzlsdslds.com