Single cell algae cultivation method (1)
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June 25 12:00:38, 2025
Single-cell algae, also known as microalgae, are tiny unicellular organisms that have gained significant attention due to their remarkable characteristics. These include high solar energy conversion efficiency, rich nutritional content, rapid growth rates, strong environmental adaptability, and ease of cultivation. Their applications are primarily in aquaculture, where they serve as direct food sources for aquatic animal larvae such as shellfish, shrimp, crab, sea cucumber, and fish larvae. Additionally, microalgae can be used to culture live feeds like rotifers, cladocerans, and copepods, which in turn are fed to various aquatic species, making microalgae an "indirect bait" in the food chain.
Beyond their role as feed, microalgae play a crucial part in water purification. During photosynthesis, they release oxygen and absorb excess nutrients like nitrogen and phosphorus, helping to reduce eutrophication and improve water quality. This not only benefits the environment but also enhances the survival and growth rates of aquatic organisms such as shrimp, crabs, and fish.
The cultivation process of single-cell algae typically involves four main steps: sterilization of containers and tools, preparation of the culture medium, inoculation, and subsequent cultivation.
First, sterilization is essential to prevent contamination. For heat-sensitive materials like plastics and rubber, chemical disinfection methods are preferred. Common techniques include using 70% alcohol for small containers or potassium permanganate solutions for larger ones. Metal and glass tools can be sterilized by boiling or direct flame. In large-scale operations, chemical agents like sodium hypochlorite are often used to disinfect tanks and equipment.
Next, the culture medium is prepared. Seawater or freshwater must first be sterilized—either by heating, filtration, or chemical treatment. Once sanitized, it is enriched with essential nutrients to support algal growth. The specific formulation depends on the type of microalgae being cultivated, ensuring optimal conditions for development.
This process requires careful attention to detail and proper execution to ensure successful cultivation and maximize the benefits of microalgae in aquaculture and environmental management.